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ISOLATION AND VALIDATION OF [6]-SHOGAOL IN GINGER ROOT EXTRACT (ZINGIBER OFFICINALE) BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

ISOLATION AND VALIDATION OF [6]-SHOGAOL IN GINGER ROOT EXTRACT (ZINGIBER OFFICINALE) BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
Le Nguyen Tuong Vi1,2, Nguyen Ngoc Tuan3, Nguyen Kim Khanh4, Nguyen Thi Nu Trinh3 Nguyen Cuu Khoa1,2*

1Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ha Noi, Viet Nam 2Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Viet Nam 3Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City, Viet Nam 4Institute of Food Processing and Post harvest Technology, Binh Duong University, Binh Duong Province, Viet Nam

Abstract:

Ginger (Zingiber officinale) and its components have unique therapeutic effects such as anti-cancer, antioxidant, free radical blockers, antibacterial, anti-inflammatory. Among the compounds of ginger, [6]-shogaol is a promising cancer preventive compound. The purpose of this paper was to present the extraction, development and validation by high performance liquid chromatography (HPLC) assay with photodiode-array detector (PDA) for [6]-shogaol in ginger root. Dried ginger Zingiber officinale (1 kg) is extracted with EtOH 90% 60˚C in 3 hours under condition of ultrasound wave, the ratio of material and solvent is 1:5 give a crude extract (170 g). Conducting column chromatography (CC) with crude extract in n-hexane - EtOAc (50:1) to EtOAc 100% collected 7 fractions. Fraction 3 was applied CC in different mixture of solvents to give [6]-shogaol (10.5 mg). The quantitative process was carried out using reverse phase column VDSpher PUR 100 C18 (25 cm × 4.6 mm, 5 µm), the mobile phase was acetonitrile - 0.1% H3PO4. The gradient elution was as follows: 0 min 10% B, 3.5 min 18% B, 4.5 min 35% B, 6 min 40% B, 10 min 20% B; flow rate 1.0 mL/min; run time in 10 minutes; injection volume 20 µL; column temperature 25℃; wavelength at 280 nm. This HPLC method allowed for the detection of [6]-shogaol in a short run time of 10 minutes. Quantitative results showed that the process had high specificity, achieved linearity with the linear regression equation y = 166129.76x + 22743.83 and correlation coefficient R2 = 0.9997, achieved the repeatability criterion with the RSD of the concentration = 1.99%, the recovery rate accuracy was 99.30% with RSD = 1.34%, limit of detection and limit of quantification were 0.41 μg/mL and 1.25 μg/mL, respectively.

Keywords: [6]-shogaol, (E)-1-(4-hydroxy-3-methoxyphenyl) dec-4-en-3-on, Zingiber officinale, phenolic, analytical methods, HPLC, photodiode-array detector (PDA).

Journal of Analytical Sciences, 03-2022

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