ISOLATION AND VALIDATION OF [6]-SHOGAOL IN GINGER ROOT EXTRACT (ZINGIBER OFFICINALE) BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Thứ năm - 14/04/2022 04:46

ISOLATION AND VALIDATION OF [6]-SHOGAOL IN GINGER ROOT EXTRACT (ZINGIBER OFFICINALE) BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
Le Nguyen Tuong Vi^1,2, Nguyen Ngoc Tuan³, Nguyen Kim Khanh⁴, Nguyen Thi Nu Trinh³ Nguyen Cuu Khoa^1,2^*

¹Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ha Noi, Viet Nam ²Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City, Viet Nam ³Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City, Viet Nam ⁴Institute of Food Processing and Post harvest Technology, Binh Duong University, Binh Duong Province, Viet Nam

Abstract:

Ginger (Zingiber officinale) and its components have unique therapeutic effects such as anti-cancer, antioxidant, free radical blockers, antibacterial, anti-inflammatory. Among the compounds of ginger, [6]-shogaol is a promising cancer preventive compound. The purpose of this paper was to present the extraction, development and validation by high performance liquid chromatography (HPLC) assay with photodiode-array detector (PDA) for [6]-shogaol in ginger root. Dried ginger Zingiber officinale (1 kg) is extracted with EtOH 90% 60˚C in 3 hours under condition of ultrasound wave, the ratio of material and solvent is 1:5 give a crude extract (170 g). Conducting column chromatography (CC) with crude extract in n-hexane - EtOAc (50:1) to EtOAc 100% collected 7 fractions. Fraction 3 was applied CC in different mixture of solvents to give [6]-shogaol (10.5 mg). The quantitative process was carried out using reverse phase column VDSpher PUR 100 C18 (25 cm × 4.6 mm, 5 µm), the mobile phase was acetonitrile - 0.1% H₃PO₄. The gradient elution was as follows: 0 min 10% B, 3.5 min 18% B, 4.5 min 35% B, 6 min 40% B, 10 min 20% B; flow rate 1.0 mL/min; run time in 10 minutes; injection volume 20 µL; column temperature 25℃; wavelength at 280 nm. This HPLC method allowed for the detection of [6]-shogaol in a short run time of 10 minutes. Quantitative results showed that the process had high specificity, achieved linearity with the linear regression equation y = 166129.76x + 22743.83 and correlation coefficient R²= 0.9997, achieved the repeatability criterion with the RSD of the concentration = 1.99%, the recovery rate accuracy was 99.30% with RSD = 1.34%, limit of detection and limit of quantification were 0.41 μg/mL and 1.25 μg/mL, respectively.

Keywords: [6]-shogaol, (E)-1-(4-hydroxy-3-methoxyphenyl) dec-4-en-3-on, Zingiber officinale, phenolic, analytical methods, HPLC, photodiode-array detector (PDA).

Journal of Analytical Sciences, 03-2022

Tác giả: admin_cntp